In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development

5.9 Acridine Orange/Ethidium Bromide Staining -- 5.10 Mitochondrial Membrane Potential Assay -- 5.11 Chromatin Condensation Assay -- 5.12 APOPercentageTM Apoptotic Assay -- 5.13 PARP Cleavage Assay -- 5.14 Flow Cytometry -- References -- Chapter 6: Cell Migratory Assays -- 6.1 Introduction -- 6.2 2D Cell Migration Assays -- 6.2.1 Wound Healing Assay -- 6.2.2 Cell Exclusion Zone Assay -- 6.2.3 Transwell Migration Assay -- 6.2.4 Fence Assay -- 6.2.5 Microfluidic Assay -- 6.2.6 Single Cell Motility Assay -- 6.3 2D/3D Migration Assays -- 6.3.1 Microcarrier Bead Assay -- 6.3.2 Spheroid Migration Assay -- 6.3.2.1 3D-Spheroid Formation Protocol -- References -- Chapter 7: In Vitro Cell Invasion Assays -- 7.1 Introduction -- 7.2 Transwell Invasion Assay -- 7.3 Platypus Invasion Assay -- 7.4 Gelatin Degradation Assay -- 7.5 3D Cell Tracking Assay -- 7.6 Vertical Gel 3D Invasion Assay -- 7.7 Spheroid/Monodispersed Cell Invasion Assay -- 7.8 Spheroid Confrontation Assay -- 7.9 Spheroid Gel Invasion Assays -- References -- Chapter 8: Anti-Angiogenesis Assays -- 8.1 Introduction -- 8.2 Endothelial Tube Formation Assay -- 8.3 Rat Aortic Ring Assay -- References -- Chapter 9: High-Throughput Screening Assays -- 9.1 Introduction -- 9.2 Cell Culture -- 9.3 High-Throughput Cytotoxicity and Apoptosis Screening in 2D Cultures -- 9.4 3D Cell-Based HTS Assays -- References -- Index